PU6::klp-12_sgRNA
(Plasmid #46170)

• Depositing Lab: John Calarco
• Publication: Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/nmeth.2532.

Backbone

• Vector backbone: pUC57
• Backbone size w/o insert (bp): 2641
• Total vector size (bp): 3481
• Vector type: Worm Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: klp-12 targeting sgRNA
• Alt name: klp-12
• Species: Synthetic
• Insert Size (bp): 840
• Entrez Gene: klp-12 (a.k.a. CELE_T01G1.1a)
• Promoter: C. elegans U6 snRNA pol III promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: M13 (-40) forward
• 3′ sequencing primer: M13 (-48) reverse

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information on Calarco Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/calarco/

gRNA target sequence GATCCACAAGTTACAATTGG