pQUASp-RedStinger (nlsRFP)
(Plasmid
#46165)
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Purpose(Empty Backbone) Expression of RedStinger in insect
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 46165 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepQUASp
- Backbone size (bp) 9929
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Vector typeInsect Expression
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Selectable markerswhite
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRedStinger
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Alt namenlsRFP
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Alt namenuclearRFP
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Alt namenlsDsRed
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Insert Size (bp)821
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer TTTGAAAACCGGTGATAGAGCCTG
- 3′ sequencing primer ACCAGGGGATGCTTAATTGTGTTC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQUASp-RedStinger (nlsRFP) was a gift from Christopher Potter (Addgene plasmid # 46165 ; http://n2t.net/addgene:46165 ; RRID:Addgene_46165)