pJC-TALE-VP64
(Plasmid
#46147)
-
Purpose(Empty Backbone)
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 46147 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepJFRC-7
-
Backbone manufacturerGerry Rubin
- Backbone size (bp) 8151
-
Modifications to backboneSubcloned in a Golden-Gate compatible TALE effector, fused with a VP64 activation domain.
-
Vector typeInsect Expression
- Promoter hsp70
-
Tag
/ Fusion Protein
- VP64 activation domain (C terminal on backbone)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pJC-TALE-VP64 was a gift from David Stern (Addgene plasmid # 46147 ; http://n2t.net/addgene:46147 ; RRID:Addgene_46147) -
For your References section:
TALE-mediated modulation of transcriptional enhancers in vivo. Crocker J, Stern DL. Nat Methods. 2013 Aug;10(8):762-7. doi: 10.1038/nmeth.2543. Epub 2013 Jun 30. 10.1038/nmeth.2543 PubMed 23817068
