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Addgene

pJC-TALE-hairy
(Plasmid #46145)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46145 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJFRC-7
  • Backbone manufacturer
    Gerry Rubin
  • Backbone size (bp) 8151
  • Modifications to backbone
    Subcloned in a Golden-Gate compatible TALE effector, fused with a Drosophila hairy repression domain.
  • Vector type
    Insect Expression
  • Promoter hsp70
  • Tag / Fusion Protein
    • Hairy Repression Domain (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJC-TALE-hairy was a gift from David Stern (Addgene plasmid # 46145 ; http://n2t.net/addgene:46145 ; RRID:Addgene_46145)

  • For your References section:

    TALE-mediated modulation of transcriptional enhancers in vivo. Crocker J, Stern DL. Nat Methods. 2013 Aug;10(8):762-7. doi: 10.1038/nmeth.2543. Epub 2013 Jun 30. 10.1038/nmeth.2543 PubMed 23817068
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