pFT1.0
(Plasmid #46138)

• Depositing Lab: Wenbiao Chen
• Publication: Ni et al Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15389-94. Epub 2012 Aug 20.

Backbone

• Vector backbone: pBluescript II KS+
• Backbone manufacturer: stratagene
• Total vector size (bp): 6131
• Vector type: Cre/Lox ; mutagenic gene trap for zebrafish

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry
• Insert Size (bp): 3900

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: DraIII (unknown if destroyed)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: mCherryR
• 3′ sequencing primer: M13 F20

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

mCherry is in the "even" or "0" reading frame starting from the exon.
This is a Tol2-based FlExTrap vector. It consists of a highly mutagenic gene trap contained within the FlEx cassette that can be stably inverted by both Cre and Flp recombinases. FRT/F3 and loxP/lox5171 are incompatible recombination sites used for the Flp and Cre recombinases, respectively, to stably invert the cassette. The reporter gene, mCherry, has its own initiation codon.