pQUAST-GAL80
(Plasmid #46137)

• Depositing Lab: Christopher Potter
• Publication: Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250.

Backbone

• Vector backbone: pQUAST
• Backbone size w/o insert (bp): 8938
• Total vector size (bp): 10260
• Vector type: Insect Expression
• Selectable markers: white

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GAL80
• Alt name: GAL4 inhibitor GAL80
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1322
• Entrez Gene: GAL80 (a.k.a. YML051W)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: Pry1
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • pQUAST-GAL80.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see this additional reference https://www.ncbi.nlm.nih.gov/pubmed/20434990 for more information on the Q-system.