pCasper4-QF#7-hsp70
(Plasmid
#46135)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 46135 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCaSpeR4
- Backbone size w/o insert (bp) 7865
- Total vector size (bp) 9182
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Modifications to backboneIntroduced hsp70 terminator
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Vector typeInsect Expression
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Selectable markerswhite
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameQF#7
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Alt nameQF DNA binding domain
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Alt nameQF Activation Domain
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Alt nameQF2
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SpeciesSynthetic
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Insert Size (bp)1053
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer pCasper-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please see this additional reference https://www.ncbi.nlm.nih.gov/pubmed/20434990 for more information on the Q-system.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCasper4-QF#7-hsp70 was a gift from Christopher Potter (Addgene plasmid # 46135 ; http://n2t.net/addgene:46135 ; RRID:Addgene_46135) -
For your References section:
Improved and expanded Q-system reagents for genetic manipulations. Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ. Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250. 10.1038/nmeth.3250 PubMed 25581800