pattB-hsp70P-QF#7-hsp70T
(Plasmid #46134)

• Depositing Lab: Christopher Potter
• Publication: Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250.

Backbone

• Vector backbone: pattB
• Backbone size w/o insert (bp): 7400
• Total vector size (bp): 10623
• Modifications to backbone: Introduces hsp70 promoter
• Vector type: Insect Expression
• Selectable markers: white

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: QF#7
• Alt name: QF DNA binding domain
• Alt name: QF Activation Domain
• Alt name: QF2
• Species: Synthetic
• Insert Size (bp): 1053

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: AatII (not destroyed)
• 5′ sequencing primer: pCasper-hs

Resource Information

• Supplemental Documents:
  • pattB-hsp70P-QF#7-hsp70T.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Alternative transformation vectors are available (pCasper4-QF#7-hsp70, pPTQF#7-hsp70 and pattB-synaptobrevin-7-QFBDAD-hsp70). Please see Addgene plasmid #'s 46135, 46136, and 46115 for more information:
http://www.addgene.org/46135/
http://www.addgene.org/46136/
http://www.addgene.org/46115/

Please see this additional reference https://www.ncbi.nlm.nih.gov/pubmed/20434990 for more information on the Q-system.