pGMR-QF#7m1
(Plasmid
#46130)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 46130 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGMR
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Backbone manufacturerBruce Hay
- Backbone size w/o insert (bp) 8859
- Total vector size (bp) 9922
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Vector typeInsect Expression
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Selectable markerswhite
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameQF#7m1
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Alt nameQF#7 activation domain m1
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Alt nameQF DNA binding domain
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Alt nameQF2 with modified activation domain 1
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SpeciesSynthetic
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Insert Size (bp)1062
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer pCasper-hs (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that Addgene's sequencing results found a single nucleotide deletion of bp# 6883 when compared to the full plasmid sequence. The depositing laboratory states that this difference is not a concern for the function of the plasmid.
Please see this additional reference https://www.ncbi.nlm.nih.gov/pubmed/20434990 for more information on the Q-system.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGMR-QF#7m1 was a gift from Christopher Potter (Addgene plasmid # 46130 ; http://n2t.net/addgene:46130 ; RRID:Addgene_46130) -
For your References section:
Improved and expanded Q-system reagents for genetic manipulations. Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ. Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250. 10.1038/nmeth.3250 PubMed 25581800