pCNCre3
(Plasmid #46088)

• Depositing Lab: Raymond Monnat
• Publication: Monnat RJ et al Biochem Biophys Res Commun. 1999 Feb 5;255(1):88-93.

Backbone

• Vector backbone: pCMVbeta
• Backbone manufacturer: clontech
• Total vector size (bp): 4635
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CreI
• Species: Chlamydomonas reinhardtii
• Insert Size (bp): 521
• Promoter: CMV
• Tag / Fusion Protein:
  • NLS (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (destroyed during cloning)
• 3′ cloning site: NotI (destroyed during cloning)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EBV-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The I-CreI expression vector pCNCre3 was constructed from plasmid pI-CreI (Wang, et al., 1997. Nucleic Acids Res. 25, 3767–3776). The I-CreI open reading frame was amplified, cleaved with HhaI and then ligated to the adapter oligonucleotide described above. The resulting nls-Cre fragment was ligated into the NotI site of pCMVb to generate pCNCre3.