pCNPpo6-H98A
(Plasmid
#46087)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 46087 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMVbeta
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Backbone manufacturerclontech
- Total vector size (bp) 4255
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePpoI H98A
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Alt namePpoI
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Speciesphysarum polycephalum
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Insert Size (bp)515
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Mutationcatalytically inactive H98A variant
- Promoter CMV
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Tag
/ Fusion Protein
- NLS (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (destroyed during cloning)
- 3′ cloning site NotI (destroyed during cloning)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See Addgene plasmid 46086 for description of wild type plasmid.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCNPpo6-H98A was a gift from Raymond Monnat (Addgene plasmid # 46087 ; http://n2t.net/addgene:46087 ; RRID:Addgene_46087) -
For your References section:
Generation of highly site-specific DNA double-strand breaks in human cells by the homing endonucleases I-PpoI and I-CreI. Monnat RJ Jr, Hackmann AF, Cantrell MA. Biochem Biophys Res Commun. 1999 Feb 5;255(1):88-93. 10.1006/bbrc.1999.0152 PubMed 10082660
