pCNPpo6
(Plasmid #46086)

• Depositing Lab: Raymond Monnat
• Publication: Monnat RJ et al Biochem Biophys Res Commun. 1999 Feb 5;255(1):88-93.

Backbone

• Vector backbone: pCMVbeta
• Backbone manufacturer: clontech
• Total vector size (bp): 4255
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PpoI
• Species: physarum polycephalum
• Insert Size (bp): 515
• Promoter: CMV
• Tag / Fusion Protein:
  • NLS (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (destroyed during cloning)
• 3′ cloning site: NotI (destroyed during cloning)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EBV-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The I-PpoI expression vector pCNPpo6 was constructed from a cloned Physarum intron (pI3-941). The I-PpoI ORF was PCR-amplified, digested with HhaI and then ligated to an oligonucleotide adapter to add a new ATG start codon, an in-frame, N-terminal SV40 large T-antigen nuclear localization signal (nls)and flanking restriction cleavage sites. The resulting nls-Ppo fragment was ligated into the NotI site of pCMVbeta to generate pCNPpo6.