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Addgene

pGEMT-Map7-856-1351
(Plasmid #46069)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46069 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM-T
  • Total vector size (bp) 3513
  • Vector type
    Bacterial Expression, Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Map7-856-1351
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    496
  • GenBank ID
    BC052637.1
  • Entrez Gene
    Map7 (a.k.a. E-MAP-115, MAP-7, Mtap7, mshi, mste, ste)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer M13-F20
  • 3′ sequencing primer M13-Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Discrepancies between the Addgene QC sequence and full sequence do not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEMT-Map7-856-1351 was a gift from Edgar Gomes (Addgene plasmid # 46069 ; http://n2t.net/addgene:46069 ; RRID:Addgene_46069)

  • For your References section:

    MAP and kinesin-dependent nuclear positioning is required for skeletal muscle function. Metzger T, Gache V, Xu M, Cadot B, Folker ES, Richardson BE, Gomes ER, Baylies MK. Nature. 2012 Mar 18;484(7392):120-4. doi: 10.1038/nature10914. 10.1038/nature10914 PubMed 22425998
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