pT3-EF1a-NICD1
(Plasmid #46047)

• Depositing Lab: Xin Chen
• Publication: Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5. doi: 10.1172/JCI63212. Epub 2012 Jul 17.

Backbone

• Vector backbone: pT3-EF1a
• Backbone size w/o insert (bp): 7000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Notch1 (C-term)
• Alt name: Notch 1
• Species: M. musculus (mouse)
• Insert Size (bp): 2385
• Mutation: Cytoplasmic domain initiating at amino acid 1753
• GenBank ID:
• Entrez Gene: Notch1 (a.k.a. 9930111A19Rik, Mis6, N1, Tan1, lin-12)
• Promoter: EF1a
• Tag / Fusion Protein:
  • Myc tag (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: Notch 1 from Addgene plasmid 15131: pLIA mNIC myc.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is in the pT3-EF1a vector which contains loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid cannot not used with Cre for in vivo studies.

For in vivo use in mice with Cre, please use plasmid 86500.