tetO-Hand2
(Plasmid #46028)

• Depositing Lab: John Gearhart
• Publication: Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-106. doi: 10.1016/j.yjmcc.2013.04.004.

Backbone

• Vector backbone: tetO-Gateway
• Total vector size (bp): 10000
• Modifications to backbone: Hand2 cloned into Gateway site of tetO-Gateway
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Zeo marker is outside the LTRs and will not be packaged into virus.

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Hand2
• Species: M. musculus (mouse)
• Insert Size (bp): 715
• Entrez Gene: Hand2 (a.k.a. AI225906, AI661148, Ehan, Ehand2, Hed, Th2, Thin, Thing2, bHLHa, bHLHa26, dHA, dHAND)
• Promoter: tetO

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry