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Addgene

pOSIP-KT (KanR, P21) Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pOSIP-KT (KanR, P21) with the following enzymes: SacI and NotI-HF.

Digests with SacI and SacI/NotI-HF show the correct band sizes:

SacI (expected band 6.8 kb)
SacI/NotI-HF (expected bands 1.1 kb, 2.2kb and 3.4kb)