pE-FLP
(Plasmid #45978)

• Depositing Labs: Drew Endy, Keith Shearwin
• Publication: St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.

Backbone

• Vector backbone: pKD46
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): E811 (P2 lysogen)
• Growth instructions: For propagation, please grow this plasmid in the original strain (E811) In that strain, the strong promoter pE will be repressed, thus avoiding possible toxicity and accumulation of unwanted mutations in the plasmid. Use 50ug/ml ampicillin (100ug/ml may inhibit growth)
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: flp
• Alt name: flippase
• Insert Size (bp): 1272
• Mutation: flp driven by the constitutive promoter pE from phage P2
• Promoter: pE (from phage P2)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: GGAATAAGGGCGACACGGAAATGTTGAATA
• 3′ sequencing primer: CCGTTACATATCAAAGGGAAAACTGTCC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The flippase gene was amplified from pCP20 (e.g. available from the Coli Genetic Stock Center)
• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Can be used to remove the integration module (including the antibiotic resistance cassette) from pOSIP integrants.

Reference:
St-Pierre F, Cui L et al., "One-step cloning and chromosomal integration of DNA", ACS Synthetic Biology, http://pubs.acs.org/doi/abs/10.1021/sb400021j