pGEX-parkin 219-465
(Plasmid #45972)

• Depositing Lab: Kalle Gehring
• Publication: Trempe et al Science. 2013 May 9.

Backbone

• Vector backbone: pGEX-6P-1
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 4966
• Total vector size (bp): 5707
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Parkin 219-465
• Alt name: PARK2
• Species: R. norvegicus (rat)
• Insert Size (bp): 741
• Mutation: Codon usage optimized for E.coli expression. Deleted a.a. 1-219
• Entrez Gene: Prkn (a.k.a. Park, Park2)
• Promoter: tac
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: PGEXF
• 3′ sequencing primer: PGEXR

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Véronique Sauvé performed PCR amplification from WT codon-optimized gene and subcloned it.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry