pENTR-L4-UBI-L1R
(Plasmid #45959)

• Depositing Lab: Patrick Salmon
• Publication: Giry-Laterriere et al Methods Mol Biol. 2011;737:183-209. doi: 10.1007/978-1-61779-095-9_8.

Backbone

• Vector backbone: pDONRP4P1R
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4777
• Total vector size (bp): 3878
• Vector type: pENTR plasmid

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human UBI promoter
• Alt name: Ubiquitin c promoter
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1221
• Entrez Gene: UBC (a.k.a. HMG20)
• Promoter: Ubiquitin

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13f
• 3′ sequencing primer: M13r

Resource Information

• Supplemental Documents:
  • pCWX PG PC gateway system
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

More information can be found at http://medweb2.unige.ch/salmon/lentilab/lentivectors.html

NOTE: There are some discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These differences should not affect plasmid function.