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pENTR-L4-pTF-L1R
(Plasmid #45954)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45954 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONRP4P1R
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4777
  • Total vector size (bp) 2955
  • Modifications to backbone
    BP reaction with B4B1R pTF promoter amplicon.
  • Vector type
    Gateway Donor Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pTF promoter
  • Alt name
    optimized TET promoter
  • Species
    Synthetic
  • Insert Size (bp)
    312
  • Promoter TET optimized

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-L4-pTF-L1R was a gift from Patrick Salmon (Addgene plasmid # 45954 ; http://n2t.net/addgene:45954 ; RRID:Addgene_45954)

  • For your References section:

    Polyswitch lentivectors: "all-in-one" lentiviral vectors for drug-inducible gene expression, live selection, and recombination cloning. Giry-Laterriere M, Cherpin O, Kim YS, Jensen J, Salmon P. Hum Gene Ther. 2011 Oct;22(10):1255-67. Epub 2011 Sep 27. 10.1089/hum.2010.179 PubMed 21761975
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