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Addgene

pEGFPN1-Hs beta1
(Plasmid #45927)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45927 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 7514
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Hs Beta1 adaptin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2814
  • Entrez Gene
    AP1B1 (a.k.a. ADTB1, AP105A, BAM22, CLAPB2, KIDAR)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EGFP-N Sequencing Primer (#6479-1)
  • 3′ sequencing primer EGFP-C Sequencing Primer (#6478-1)
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    From Dr. Juan Bonifacino’s lab at NIH: Hs beta1/pGADT7

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFPN1-Hs beta1 was a gift from James Hurley (Addgene plasmid # 45927 ; http://n2t.net/addgene:45927 ; RRID:Addgene_45927)

  • For your References section:

    Structural basis for recruitment and activation of the AP-1 clathrin adaptor complex by Arf1. Ren X, Farias GG, Canagarajah BJ, Bonifacino JS, Hurley JH. Cell. 2013 Feb 14;152(4):755-67. doi: 10.1016/j.cell.2012.12.042. 10.1016/j.cell.2012.12.042 PubMed 23415225
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