YCplac111-yVps23-mCherry
(Plasmid
#45925)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45925 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneYCplac111
- Backbone size w/o insert (bp) 6103
- Total vector size (bp) 8601
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameyeast Vps23
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)2498
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Entrez GeneSTP22 (a.k.a. YCL008C, AGS1, VPL15, VPS23)
- Promoter lac
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Tag
/ Fusion Protein
- mCherry (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer M13_pUC_rev; M13-rev
- 3′ sequencing primer mCherry-R; M13_pUC_fwd; M13-F20 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YCplac111-yVps23-mCherry was a gift from James Hurley (Addgene plasmid # 45925 ; http://n2t.net/addgene:45925 ; RRID:Addgene_45925) -
For your References section:
Structural basis for endosomal recruitment of ESCRT-I by ESCRT-0 in yeast. Ren X, Hurley JH. EMBO J. 2011 Jun 1;30(11):2130-9. doi: 10.1038/emboj.2011.122. Epub 2011 Apr 19. 10.1038/emboj.2011.122 PubMed 21505419