Skip to main content
Addgene

pGST2-yVps23-UEV_C133A
(Plasmid #45924)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45924 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGST-parallel.2
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 5480
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    yeast Vps23-UEV(1-160)
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    480
  • Mutation
    C133A
  • Entrez Gene
    STP22 (a.k.a. YCL008C, AGS1, VPL15, VPS23)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGST2-yVps23-UEV_C133A was a gift from James Hurley (Addgene plasmid # 45924 ; http://n2t.net/addgene:45924 ; RRID:Addgene_45924)

  • For your References section:

    Structural basis for endosomal recruitment of ESCRT-I by ESCRT-0 in yeast. Ren X, Hurley JH. EMBO J. 2011 Jun 1;30(11):2130-9. doi: 10.1038/emboj.2011.122. Epub 2011 Apr 19. 10.1038/emboj.2011.122 PubMed 21505419
Related items: