FLAG-SNAP29
(Plasmid #45915)

• Depositing Lab: Noboru Mizushima
• Publication: Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10.1016/j.cell.2012.11.001.

Backbone

• Vector backbone: CMV10 FLAG
• Backbone size w/o insert (bp): 6400
• Total vector size (bp): 7174
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: synaptosomal-associated protein, 29kDa
• Alt name: SNAP29
• Species: H. sapiens (human)
• Insert Size (bp): 774
• GenBank ID: NM_004782.3
• Entrez Gene: SNAP29 (a.k.a. CEDNIK, SNAP-29)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry