FLAG-VAMP7
(Plasmid #45913)

• Depositing Lab: Noboru Mizushima
• Publication: Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10.1016/j.cell.2012.11.001.

Backbone

• Vector backbone: CMV10 FLAG
• Backbone size w/o insert (bp): 6400
• Total vector size (bp): 7060
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: vesicle-associated membrane protein 7
• Alt name: VAMP7
• Species: M. musculus (mouse)
• Insert Size (bp): 660
• GenBank ID: NM_011515.4
• Entrez Gene: Vamp7 (a.k.a. Sybl1, TI-VAMP, VAMP-7)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Clone 3601755; Open Biosystems, Huntsville, AL, USA
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry