pcDNA4 rat NZAP
(Plasmid
#45908)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45908 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA4
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameZc3hav1 zinc finger CCCH type, antiviral 1
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Alt nameNZAP
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Alt nameZAP
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SpeciesR. norvegicus (rat)
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Entrez GeneZc3hav1 (a.k.a. ARTD13, PARP13, Zap)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site N/A (unknown if destroyed)
- 3′ cloning site N/A (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA4 rat NZAP was a gift from Harmit Malik (Addgene plasmid # 45908 ; http://n2t.net/addgene:45908 ; RRID:Addgene_45908) -
For your References section:
Positive selection and increased antiviral activity associated with the PARP-containing isoform of human zinc-finger antiviral protein. Kerns JA, Emerman M, Malik HS. PLoS Genet. 2008 Jan;4(1):e21. doi: 10.1371/journal.pgen.0040021. 10.1371/journal.pgen.0040021 PubMed 18225958