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Addgene

pcDNA4 huZAP(L)
(Plasmid #45907)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45907 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ZAP(L) isoform
  • Alt name
    ZAP
  • Alt name
    ZC3HAV1 zinc finger CCCH-type, antiviral 1
  • Species
    H. sapiens (human)
  • Mutation
    M202T, L388F, Q701E, T851I (reference NP_064504.2)
  • Entrez Gene
    ZC3HAV1 (a.k.a. ARTD13, FLB6421, PARP13, ZAP, ZC3H2, ZC3HDC2)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site N/A (unknown if destroyed)
  • 3′ cloning site N/A (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-Rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is a M202T amino acid change in hZAP(L) isoform, this does not effect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4 huZAP(L) was a gift from Harmit Malik (Addgene plasmid # 45907 ; http://n2t.net/addgene:45907 ; RRID:Addgene_45907)

  • For your References section:

    Positive selection and increased antiviral activity associated with the PARP-containing isoform of human zinc-finger antiviral protein. Kerns JA, Emerman M, Malik HS. PLoS Genet. 2008 Jan;4(1):e21. doi: 10.1371/journal.pgen.0040021. 10.1371/journal.pgen.0040021 PubMed 18225958
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