pMP31-1
(Plasmid #45905)

• Depositing Lab: Harmit Malik
• Publication: Patel et al PLoS Biol. 2012;10(3):e1001282. doi: 10.1371/journal.pbio.1001282. Epub 2012 Mar 13.

Backbone

• Vector backbone: pFLAG-CMV2
• Backbone manufacturer: Sigma
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mitochondrial antiviral signaling protein
• Alt name: MAVS
• Species: H. sapiens (human)
• Insert Size (bp): 1620
• GenBank ID: 57506
• Entrez Gene: MAVS (a.k.a. CARDIF, IPS-1, IPS1, VISA)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry