pMP56-5
(Plasmid
#45904)
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45904 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepFLAG-CMV2
-
Backbone manufacturerSigma
- Backbone size w/o insert (bp) 4700
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namemitochondrial antiviral signaling protein, mutation C508R
-
Alt nameMAVS
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1620
-
MutationC508R
-
GenBank ID57506
-
Entrez GeneMAVS (a.k.a. CARDIF, IPS-1, IPS1, VISA)
- Promoter CMV
-
Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pMP56-5 was a gift from Harmit Malik (Addgene plasmid # 45904 ; http://n2t.net/addgene:45904 ; RRID:Addgene_45904) -
For your References section:
Convergent evolution of escape from hepaciviral antagonism in primates. Patel MR, Loo YM, Horner SM, Gale M Jr, Malik HS. PLoS Biol. 2012;10(3):e1001282. doi: 10.1371/journal.pbio.1001282. Epub 2012 Mar 13. 10.1371/journal.pbio.1001282 PubMed 22427742