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Addgene

pCR2.1-SNORD116-3
(Plasmid #45891)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45891 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR2.1
  • Backbone manufacturer
    Invitrogene
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SNORD115 with 80nt from intron sequence but without flanking exons
  • Alt name
    HBII-85
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    177
  • GenBank ID
    Entrez Gene: 100033415
  • Entrez Gene
    SNORD116-3 (a.k.a. HBII-85-3)

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer GCCCTTTACCCGTGAACCAC
  • 3′ sequencing primer GCCCTTCAGCCTATGATG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR2.1-SNORD116-3 was a gift from Stefan Stamm (Addgene plasmid # 45891 ; http://n2t.net/addgene:45891 ; RRID:Addgene_45891)

  • For your References section:

    Molecular characterization of a patient presumed to have prader-willi syndrome. Falaleeva M, Sulsona CR, Zielke HR, Currey KM, de la Grange P, Aslanzadeh V, Driscoll DJ, Stamm S. Clin Med Insights Case Rep. 2013 May 5;6:79-86. doi: 10.4137/CCRep.S11510. Print 2013. PubMed 23700380
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