pEGFP-parkin WT
(Plasmid #45875)

• Depositing Lab: Edward Fon
• Publication: Trempe et al Science. 2013 May 9.

Backbone

• Vector backbone: pEGFP-C2
• Backbone size w/o insert (bp): 4731
• Total vector size (bp): 6129
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Parkin
• Alt name: PARK2
• Species: H. sapiens (human)
• Insert Size (bp): 1398
• GenBank ID: NM_004562.2
• Entrez Gene: PRKN (a.k.a. AR-JP, LPRS2, PARK2, PDJ)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: EGFP_C_primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Lab of Heidi McBride
• Articles Citing this Plasmid:
  • 22 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry