pCRII-Topo Semcap3
(Plasmid #45874)

• Depositing Lab: Jeffrey Macklis
• Publication: Macklis lab in situ probes (unpublished)

Backbone

• Vector backbone: pCRII-Topo
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 4600
• Vector type: in situ

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Semcap3 in situ probe
• Alt name: Semcap3
• Alt name: Pdzrn3
• Alt name: PDZ domain containing RING finger 3
• Species: M. musculus (mouse)
• Insert Size (bp): 620
• Mutation: contains bp# 3184-3805 of AF127085.1
• GenBank ID:
• Entrez Gene: Pdzrn3 (a.k.a. 1110020C07Rik, AI429718, AL023082, LNX3, SEMACAP3, Semcap3)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: M13 Rev, Sp6
• 3′ sequencing primer: T7, M13 Forward

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For in vitro transcription, use the NotI restriction digest and Sp6 promoter for sense probe generation and the HindIII restriction digest and T7 promoter for antisense probe generation.

Please note that Addgene's sequencing results match bp# 3184-3805 of AF127085.1, not bp# 3185-3804 as indicated on plasmid map. The plasmid functions as described in the associated publication.