pCRII-Topo Hnrp
(Plasmid #45872)

• Depositing Lab: Jeffrey Macklis
• Publication: Macklis lab in situ probes (unpublished)

Backbone

• Vector backbone: pCRII-Topo
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 4300
• Vector type: in situ

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Hnrnpa2b1 in situ probe
• Alt name: Hnrnpa2b1
• Alt name: Hnrp
• Alt name: heterogeneous nuclear ribonucleoprotein A2/B1
• Species: M. musculus (mouse)
• Insert Size (bp): 333
• Mutation: contains bp# 1145-1370 of NM_182650.3
• GenBank ID: NM_182650
• Entrez Gene: Hnrpa2b1

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: M13 Rev, Sp6
• 3′ sequencing primer: T7, M13 Forward

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For in vitro transcription, use the NotI restriction digest and Sp6 promoter for sense probe generation and the Spe1 restriction digest and T7 promoter for antisense probe generation.

Please note that Addgene's sequencing results match bp# 1145-1370 (with a single nucleotide mismatch at bp#1280) when compared to NM_182650.3, not bp# 1124-1456 as indicated on plasmid map. The plasmid functions as described in the associated publication.