pCRII-Topo Couptf1
(Plasmid
#45870)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 45870 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCRII-Topo
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 4600
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Vector typein situ
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCouptf1 in situ probe
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Alt nameCouptf1
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Alt nameNr2f1
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Alt namenuclear receptor subfamily 2, group F, member 1
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SpeciesM. musculus (mouse)
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Insert Size (bp)619
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Mutationcontains bp# 1755-2381 of NM_010151.2
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GenBank IDNM_010151
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Entrez GeneNr2f1 (a.k.a. COUP-, COUP-T, COUP-TF1, COUP-TFI, COUPTFA, EAR-3, EAR3, Erb, Erbal3, SVP44, Tcfcou, Tcfcoup1)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13 Rev, Sp6
- 3′ sequencing primer T7, M13 Forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For in vitro transcription, use the HindIII restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and Sp6 promoter for antisense probe generation.
Please note that Addgene's sequencing results match bp# 1755-2381 of NM_010151.2, not bp# 1635-2273 as indicated on plasmid map. The plasmid functions as described in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCRII-Topo Couptf1 was a gift from Jeffrey Macklis (Addgene plasmid # 45870 ; http://n2t.net/addgene:45870 ; RRID:Addgene_45870)
Map uploaded by the depositor.