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pCRII-Topo Tmtc4
(Plasmid #45869)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45869 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCRII-Topo
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 4600
  • Vector type
    in situ

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TMTC4 in situ probe
  • Alt name
    TMTC4
  • Alt name
    j22riken
  • Alt name
    transmembrane and tetratricopeptide repeat containing 4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    579
  • Mutation
    nt 1220-1798 on BC031368
  • GenBank ID
    NM_028651
  • Entrez Gene
    Tmtc4 (a.k.a. 4930403J22Rik, 5730419O14Rik)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Tmtc4 fragment was amplified by RT-PCR using the following primer pair:
GAAGCAGAGCAGAGCTACCG
TCTGAACAGAGGCTTCATGC
For in vitro transcription, use the Spe1 restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and Sp6 promoter for antisense probe generation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRII-Topo Tmtc4 was a gift from Jeffrey Macklis (Addgene plasmid # 45869 ; http://n2t.net/addgene:45869 ; RRID:Addgene_45869)

  • For your References section:

    Novel subtype-specific genes identify distinct subpopulations of callosal projection neurons. Molyneaux BJ, Arlotta P, Fame RM, MacDonald JL, MacQuarrie KL, Macklis JD. J Neurosci. 2009 Sep 30;29(39):12343-54. doi: 10.1523/JNEUROSCI.6108-08.2009. 10.1523/JNEUROSCI.6108-08.2009 PubMed 19793993
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