pCM157
(Plasmid #45863)

• Depositing Lab: Mary Lidstrom
• Publication: Marx et al Biotechniques. 2002 Nov;33(5):1062-7.

Backbone

• Vector backbone: pCM62
• Backbone manufacturer: Lidstrom Lab (Addgene plasmid 45826)
• Backbone size w/o insert (bp): 6878
• Total vector size (bp): 7978
• Vector type: Bacterial Expression, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Tetracycline, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cre recombinase
• Alt name: cyclization recombinase
• Alt name: cre
• Species: Enterobacteria phage P1
• Insert Size (bp): 1100
• Promoter: lac

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: M13-F20
• 3′ sequencing primer: M13R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The 1.1-kb XbaI-EcoRI fragment from pJW168 (PMID 9858684) was cloned between the XbaI and EcoRI sites of pCM62 (Addgene plasmid 45826) to generate the tetracycline-resistance conferring cre expression plasmid.
Used with allelic exhange vectors--Addgene plasmids 46012 or 46013--to generate unmarked mutant strains in a broad bacterial host range.