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Addgene

Tet-O-FUW-Nkx6.1
(Plasmid #45846)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45846 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Tet-O-FUW
  • Total vector size (bp) 9478
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nkx6.1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1098
  • GenBank ID
    NM_144955.2
  • Entrez Gene
    Nkx6-1 (a.k.a. NKX6A, Nkx6.1)
  • Promoter tet-ON

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer LNCX
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tet-O-FUW-Nkx6.1 was a gift from Marius Wernig (Addgene plasmid # 45846 ; http://n2t.net/addgene:45846 ; RRID:Addgene_45846)

  • For your References section:

    Generation of oligodendroglial cells by direct lineage conversion. Yang N, Zuchero JB, Ahlenius H, Marro S, Ng YH, Vierbuchen T, Hawkins JS, Geissler R, Barres BA, Wernig M. Nat Biotechnol. 2013 May;31(5):434-9. doi: 10.1038/nbt.2564. Epub 2013 Apr 14. 10.1038/nbt.2564 PubMed 23584610
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