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Addgene

pMSCV-puro-AS9 (Mito MTS)-Delta N67-mMCL1
(Plasmid #45822)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45822 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCV-puro
  • Backbone size w/o insert (bp) 6295
  • Total vector size (bp) 7297
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AS9(Mito MTS)-Delta N67-mMCL1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1002
  • Mutation
    ATP Synthase subunit 9 is used as a Mito Targeting sequence and fused to Delta N67-mMCL-1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer 5' primer (1333–1355): 5'-CCCTTGAACCTCCTCGTTCGACC-3'
  • 3′ sequencing primer 3' primer (1660–1682): 5'-GAGACGTGCTACTTCCATTTGTC-3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-puro-AS9 (Mito MTS)-Delta N67-mMCL1 was a gift from Joseph Opferman (Addgene plasmid # 45822 ; http://n2t.net/addgene:45822 ; RRID:Addgene_45822)

  • For your References section:

    Anti-apoptotic MCL-1 localizes to the mitochondrial matrix and couples mitochondrial fusion to respiration. Perciavalle RM, Stewart DP, Koss B, Lynch J, Milasta S, Bathina M, Temirov J, Cleland MM, Pelletier S, Schuetz JD, Youle RJ, Green DR, Opferman JT. Nat Cell Biol. 2012 Apr 29;14(6):575-83. doi: 10.1038/ncb2488. 10.1038/ncb2488 PubMed 22544066
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