pMSCV-puro-5A, 6A mMCL1-OM
(Plasmid
#45818)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45818 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMSCV-puro
- Backbone size w/o insert (bp) 6295
- Total vector size (bp) 7291
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name5A, 6A mMCL-1-OM
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SpeciesM. musculus (mouse)
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Insert Size (bp)996
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MutationChanged Arginine to Alanine at 5th and 6th amino acids
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer 5' primer (1333–1355): 5'-CCCTTGAACCTCCTCGTTCGACC-3'
- 3′ sequencing primer 3' primer (1660–1682): 5'-GAGACGTGCTACTTCCATTTGTC-3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSCV-puro-5A, 6A mMCL1-OM was a gift from Joseph Opferman (Addgene plasmid # 45818 ; http://n2t.net/addgene:45818 ; RRID:Addgene_45818) -
For your References section:
Anti-apoptotic MCL-1 localizes to the mitochondrial matrix and couples mitochondrial fusion to respiration. Perciavalle RM, Stewart DP, Koss B, Lynch J, Milasta S, Bathina M, Temirov J, Cleland MM, Pelletier S, Schuetz JD, Youle RJ, Green DR, Opferman JT. Nat Cell Biol. 2012 Apr 29;14(6):575-83. doi: 10.1038/ncb2488. 10.1038/ncb2488 PubMed 22544066