α1GA-HE3-pcDNA3
(Plasmid #45812)

• Depositing Lab: Edward Perez-Reyes
• Publication: Perez-Reyes unpublished plasmids (unpublished)

Backbone

• Vector backbone: pcDNA3-HE3
• Backbone manufacturer: Perez-Reyes
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 12700
• Modifications to backbone: pcDNA3 has been modified to contain 54 bp of the 5
  untranslated region of Xenopus beta globin. Previous studies have shown that this sequence can significantly enhance expression in Xenopus oocytes (Liman et al., 1992), and does not interfere with expression in mammalian cells (Lee et al., 1999).
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: α1Ga
• Alt name: CACNA1G
• Alt name: Cav3.1
• Alt name: calcium channel, voltage-dependent, T type, alpha 1G subunit
• Species: R. norvegicus (rat)
• Insert Size (bp): 7200
• GenBank ID: AF027984
• Entrez Gene: Cacna1g
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (destroyed during cloning)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: CMV-F; T7
• 3′ sequencing primer: BGHrev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Identification of Cav3.1 is described in Nature. 1998 Feb 26;391(6670):896-900. Note that there are some differences compared to GenBank AF027984 (H1061L, deletion of amino acid L at 1700, H1735D, F2149S and F2170S) which was sequenced manually at the time. The Addgene NGS sequence is accurate.