hα1Ga-pDsRed
(Plasmid #45811)

• Depositing Lab: Edward Perez-Reyes
• Publication: Perez-Reyes unpublished plasmids (unpublished)

Backbone

• Vector backbone: pDsRed-Express-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 11662
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: hα1Ga
• Alt name: CACNA1G
• Alt name: Cav3.1a
• Alt name: calcium channel, voltage-dependent, T type, alpha 1G subunit
• Species: H. sapiens (human)
• Insert Size (bp): 6962
• GenBank ID: AF190860
• Entrez Gene: CACNA1G (a.k.a. Ca(V)T.1, Cav3.1, NBR13, SCA42, SCA42ND)
• Promoter: CMV

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: DsRed1-N

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Cav3.1 insert retains its STOP codon, so there is no expression of the Ds-Red. The 5'UTR is gone and contains a new SalI site. The 3' end of insert ends in the endogenous Kpn1 site.
Identification of Cav3.1 is described in Nature. 1998 Feb 26;391(6670):896-900.