a1Ic-HE3-pcDNA3
(Plasmid #45810)

• Depositing Lab: Edward Perez-Reyes
• Publication: Gomora et al Biophys J. 2002 Jul;83(1):229-41.

Backbone

• Vector backbone: pcDNA3-HE3
• Backbone manufacturer: Perez-Reyes
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 13200
• Modifications to backbone: pcDNA3 has been modified to contain 54 bp of the 5
  untranslated region of Xenopus beta globin. Previous studies have shown that this sequence can significantly enhance expression in Xenopus oocytes (Liman et al., 1992), and does not interfere with expression in mammalian cells (Lee et al., 1999).
• Vector type: Mammalian Expression ; making cRNA for oocyte injections
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CACNA1I
• Alt name: Cav3.3c
• Alt name: calcium channel, voltage-dependent, T type, alpha 1I subunit
• Alt name: LT9
• Species: H. sapiens (human)
• Insert Size (bp): 7700
• GenBank ID: AF393329
• Entrez Gene: CACNA1I (a.k.a. Cav3.3, ca(v)3.3)
• Promoter: CMV

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F; T7
• 3′ sequencing primer: BGHrev

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

coding region ends at 6568