a1Ha-pcDNA3
(Plasmid #45809)

• Depositing Lab: Edward Perez-Reyes
• Publication: Cribbs et al Circ Res. 1998 Jul 13;83(1):103-9.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 13000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: a1Ha
• Alt name: CACNA1H
• Alt name: Cav3.2a
• Alt name: α1H, Hh8
• Species: H. sapiens (human)
• Insert Size (bp): 7800
• GenBank ID: AF051946
• Entrez Gene: CACNA1H (a.k.a. CACNA1HB, Cav3.2, ECA6, EIG6, HALD4)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F; T7
• 3′ sequencing primer: BGHrev

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

contains nt −74 to 7461 of α1H
5' end cloned into EcoRV site of pcDNA3: GATATCATCGATGAATTC then CGGGCGGTG as reported in Genbank AF051946.
3' ends same place as Genbank entry (CTCCCCTCCG), then a bit of the PUC18 polylinker, before the XbaI site of pcDNA3.