α1Ic-HE2-pcDNA3
(Plasmid #45808)

• Depositing Lab: Edward Perez-Reyes
• Publication: Murbartian et al FEBS Lett. 2002 Sep 25;528(1-3):272-8.

Backbone

• Vector backbone: pcDNA3-HE2
• Backbone manufacturer: Perez-Reyes
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 12400
• Modifications to backbone: pcDNA3 has been modified to contain 54 bp of the 5
  untranslated region of Xenopus beta globin. Previous studies have shown that this sequence can significantly enhance expression in Xenopus oocytes (Liman et al., 1992), and does not interfere with expression in mammalian cells (Lee et al., 1999).
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: α1Ic
• Alt name: cav3.3c
• Alt name: calcium channel, voltage-dependent, T type, alpha 1I subunit
• Alt name: Cacna1i
• Species: R. norvegicus (rat)
• GenBank ID: AY128645
• Entrez Gene: Cacna1i
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (destroyed during cloning)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F; T7
• 3′ sequencing primer: BGHrev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry