TN-XXL pcDNA3
(Plasmid
#45797)
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PurposeFluorescent reporter for calcium signaling
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45797 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 7200
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTN-XXL
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SpeciesG. gallus (chicken)
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Insert Size (bp)1885
- Promoter CMV
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Tags
/ Fusion Proteins
- ECFP (N terminal on insert)
- YFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TN-XXL pcDNA3 was a gift from Oliver Griesbeck (Addgene plasmid # 45797 ; http://n2t.net/addgene:45797 ; RRID:Addgene_45797) -
For your References section:
A genetically encoded calcium indicator for chronic in vivo two-photon imaging. Mank M, Santos AF, Direnberger S, Mrsic-Flogel TD, Hofer SB, Stein V, Hendel T, Reiff DF, Levelt C, Borst A, Bonhoeffer T, Hubener M, Griesbeck O. Nat Methods. 2008 Sep;5(9):805-11. doi: 10.1038/nmeth.1243. 10.1038/nmeth.1243 PubMed 19160515