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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 45773 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5446
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIL2R/hE-cadherin-cytotail
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Alt nameInterleukin-2 receptor α subunit extracellular and transmembrane domains
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Alt nameE-cadherin cytoplasmic domain
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SpeciesH. sapiens (human)
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MutationInterleukin-2 receptor α subunit extracellular and transmembrane domains fused to E-cadherin cytoplasmic domain
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Entrez GeneIL2RA (a.k.a. CD25, IDDM10, IL2R, IMD41, TCGFR, p55)
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Entrez GeneCDH1 (a.k.a. Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV-F; T7
- 3′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byHuman E-cadherin partial cDNA provided by David Rimm (Yale University, New Haven, CT). IL2R-1 α subunit cDNA from pCMV-IL2R-1 from Susan LaFlamme (Albany Medical College, Albany, NY), originally from Anthony Giordano (Giordano et al ., 1991)
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This IL2R/E-cadherin cytoplasmic chimera fuses the extracellular and transmembrane domains of the interleukin-2 receptor α subunit to the cytoplasmic domain of E-cadherin. This construct can bind β-catenin but cannot engage in homophilic adhesive activity.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
IL2R/hE-cadherin-cytotail was a gift from Barry Gumbiner (Addgene plasmid # 45773 ; http://n2t.net/addgene:45773 ; RRID:Addgene_45773) -
For your References section:
E-cadherin suppresses cellular transformation by inhibiting beta-catenin signaling in an adhesion-independent manner. Gottardi CJ, Wong E, Gumbiner BM. J Cell Biol. 2001 May 28;153(5):1049-60. 10.1083/jcb.153.5.1049 PubMed 11381089