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Addgene

hE-cadherin/Δp120
(Plasmid #45770)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45770 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLKpac1
  • Total vector size (bp) 8550
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hE-cadherin/Δp120
  • Alt name
    hE-cadherin764AAA
  • Alt name
    E-cadherin
  • Alt name
    CDH-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3100
  • Mutation
    Amino acids 762-764 converted from EED to AAA to abolish p120 interaction
  • GenBank ID
    NM_004360.3 NP_004351.1
  • Entrez Gene
    CDH1 (a.k.a. Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CMV-F; T7
  • 3′ sequencing primer BGH-rev; Sp6
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    E-cadherin Δp120 cDNA from plasmid pLK-hEcad 764AAA from Albert B. Reynolds (Vanderbilt University, Nashville, TN).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The three-alanine substitution present in this plasmid prevent the hE-cadherin gene product from interacting with p120 catenin.

Alternate plasmid name: hE-cadherin-762AAA-pcDNA3

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hE-cadherin/Δp120 was a gift from Barry Gumbiner (Addgene plasmid # 45770 ; http://n2t.net/addgene:45770 ; RRID:Addgene_45770)

  • For your References section:

    E-cadherin suppresses cellular transformation by inhibiting beta-catenin signaling in an adhesion-independent manner. Gottardi CJ, Wong E, Gumbiner BM. J Cell Biol. 2001 May 28;153(5):1049-60. 10.1083/jcb.153.5.1049 PubMed 11381089
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