pCAG-PLE-IRES-mCherry
(Plasmid #45766)

• Depositing Lab: Luke Lavis
• Publication: Tian et al Proc Natl Acad Sci U S A. 2012 Mar 27;109(13):4756-61. doi: 10.1073/pnas.1111943109. Epub 2012 Mar 12.

Backbone

• Vector backbone: pCAGGS
• Backbone size w/o insert (bp): 7231
• Total vector size (bp): 8944
• Modifications to backbone: Added IRES, mCherry and WPRE to pCAGGS vector
• Vector type: Mammalian Expression
• Selectable markers: mCherry

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: esterase
• Alt name: PLE
• Species: Pig
• Insert Size (bp): 1701
• Promoter: CAG

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: GAGAACGTGGTGGTGGTGG
• 3′ sequencing primer: CAGGGTCAGCCTCTCCTTG

Resource Information

• Supplemental Documents:
  • pCAG-PLE-IRES-mCherry annotated Genbank file
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid designed for coexpression of PLE
and mCherry. PLE was subcloned upstream of mCherry and separated by an internal ribosome entry site (IRES) sequence in the pCAGGS vector.