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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 45763 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBabe-puro
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Backbone manufacturerBob Weinberg
- Backbone size w/o insert (bp) 5169
- Total vector size (bp) 7765
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namelincRNA-RoR cDNA
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Alt namelincRNA-ST8SIA3 cDNA
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SpeciesH. sapiens (human)
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Insert Size (bp)2591
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GenBank IDNR_048536.1
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Entrez GeneLINC-ROR (a.k.a. ROR, lincRNA-RoR, lincRNA-ST8SIA3)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer CTTTATCCAGCCCTCAC (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note that though there are some small discrepancies between Addgene's quality control sequence and the depositor's assembled sequence, they do not affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBabe-lincRNA-RoR was a gift from George Daley (Addgene plasmid # 45763 ; http://n2t.net/addgene:45763 ; RRID:Addgene_45763) -
For your References section:
Large intergenic non-coding RNA-RoR modulates reprogramming of human induced pluripotent stem cells. Loewer S, Cabili MN, Guttman M, Loh YH, Thomas K, Park IH, Garber M, Curran M, Onder T, Agarwal S, Manos PD, Datta S, Lander ES, Schlaeger TM, Daley GQ, Rinn JL. Nat Genet. 2010 Dec;42(12):1113-7. Epub 2010 Nov 7. 10.1038/ng.710 PubMed 21057500