pCS2-NXE+mem-KillerRed
(Plasmid #45761)

• Depositing Lab: Michael Levin
• Publication: McLaughlin Lab KillerRed plasmid (unpublished)

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 4862
• Modifications to backbone: pCS2-NXE backbone: EcoNI, XmaI, and NcoI restriction sites were added to the Polylinker II region of pCS2+.
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Membrane-bound KillerRed
• Alt name: mem-KillerRed
• Alt name: KillerRed
• Species: Synthetic
• Insert Size (bp): 788
• Promoter: CMV IE94

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The KillerRed gene was graciously provided by Dr. Vladimir Korzh. It was cloned into the pCS2-NXE plasmid by Dr. Joan Lemire, of Dr. Michael Levin's lab.
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To make sense mRNA, cut with NotI and transcribe with SP6.